NFKB1 monoclonal antibody (M01), clone 2E6
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M01), clone 2E6.
Lane 1: NFKB1 transfected lysate(105.4 KDa).
Lane 2: Non-transfected lysate.
RNAi Knockdown (Antibody validated)
Western blot analysis of NFKB1 over-expressed 293 cell line, cotransfected with NFKB1 Validated Chimera RNAi ( Cat # H00004790-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFKB1 monoclonal antibody (M01), clone 2E6 (Cat # H00004790-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between IKBKB and NFKB1. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NFKB1.
Immunogen
NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (78)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M01), clone 2E6.
Lane 1: NFKB1 transfected lysate(105.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of NFKB1 over-expressed 293 cell line, cotransfected with NFKB1 Validated Chimera RNAi ( Cat # H00004790-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFKB1 monoclonal antibody (M01), clone 2E6 (Cat # H00004790-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between IKBKB and NFKB1. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — NFKB1
Entrez GeneID
4790GeneBank Accession#
BC051765Protein Accession#
AAH51765Gene Name
NFKB1
Gene Alias
DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Omim ID
164011Gene Ontology
HyperlinkGene Summary
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
DNA binding factor KBF1|NF-kappabeta|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor kappa-B, subunit 1
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Interactome
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Pathway
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Disease
- Abortion
- Acute Lung Injury
- Adenocarcinoma
- Alcoholism
- Alzheimer disease
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
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