This antibody pair set comes with matched antibody pair to detect and quantify protein level of human KIT.
Quality Control Testing:
Standard curve using recombinant protein ( H00003815-Q01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-KIT (100 ug) 2. Detection antibody: biotinylated mouse monoclonal anti-KIT, IgG2b Kappa (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Standard curve using 293T overexpression lysate (non-denatured) as an analyte. Sandwich ELISA detection sensitivity ranging from approximately 27x to 3x dilution of the 293T overexpression lysate (non-denatured).
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq