ATF2 monoclonal antibody (M01), clone 4C12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ATF2 expression in transfected 293T cell line by ATF2 monoclonal antibody (M01), clone 4C12.
Lane 1: ATF2 transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ATF2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (48.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ATF2.
Immunogen
ATF2 (AAH26175, 1 a.a. ~ 209 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKFKLHVNSARQYKDLWNMSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPSVVETTHQDSPLPHPESTTSDEKEVPLAQTAQPTSAIVRPASLQVPNVLLTSSDSSVIIQQAVPSPTSSTVITQAPSSNRPIV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (99)
Isotype
IgG3 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (48.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ATF2 expression in transfected 293T cell line by ATF2 monoclonal antibody (M01), clone 4C12.
Lane 1: ATF2 transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ATF2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — ATF2
Entrez GeneID
1386GeneBank Accession#
BC026175Protein Accession#
AAH26175Gene Name
ATF2
Gene Alias
CRE-BP1, CREB2, HB16, MGC111558, TREB7
Gene Description
activating transcription factor 2
Omim ID
123811Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000163262|activating transcription factor 2 splice variant ATF2-var2|cAMP responsive element binding protein 2, formerly
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Interactome
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Pathway
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Disease
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