ART3 monoclonal antibody (M05), clone 3A2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ART3.
Immunogen
ART3 (NP_001170, 29 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAF
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ART3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 2 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ART3 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — ART3
Entrez GeneID
419GeneBank Accession#
NM_001179Protein Accession#
NP_001170Gene Name
ART3
Gene Alias
FLJ26404
Gene Description
ADP-ribosyltransferase 3
Omim ID
603086Gene Ontology
HyperlinkGene Summary
ADP-ribosylation is a reversible posttranslational modification used to regulate protein function. ADP-ribosyltransferases, such as ART3, transfer ADP-ribose from NAD+ to the target protein, and ADP-ribosylhydrolases (see PARG; MIM 603501) reverse the reaction (Glowacki et al., 2002 [PubMed 12070318]).[supplied by OMIM
Other Designations
mono-ADP-ribosyltransferase
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Interactome
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Disease
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Publication Reference
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Genome-Wide Expression of Azoospermia Testes Demonstrates a Specific Profile and Implicates ART3 in Genetic Susceptibility.
Okada H, Tajima A, Shichiri K, Tanaka A, Tanaka K, Inoue I.
PLoS Genetics 2008 Feb; 4(2):e26.
Application:IHC-P, Human, Human Testes from patients with non-obstructive azoospermia (NOA) and obstructive azoospermia (OA).
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Genome-Wide Expression of Azoospermia Testes Demonstrates a Specific Profile and Implicates ART3 in Genetic Susceptibility.
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