ARAF purified MaxPab rabbit polyclonal antibody (D01P)
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More Files
- More Functions
Western Blot (Tissue lysate)
ARAF MaxPab rabbit polyclonal antibody. Western Blot analysis of ARAF expression in human placenta.
Western Blot (Transfected lysate)
Western Blot analysis of ARAF expression in transfected 293T cell line (H00000369-T03) by ARAF MaxPab polyclonal antibody.
Lane 1: ARAF transfected lysate(67.60 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with anti-ARAF rabbit purified polyclonal 1:1200 and anti-BRAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to ARAF on HeLa cell. [antibody concentration 30 ug/ml]
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human ARAF protein.
Immunogen
ARAF (NP_001645.1, 1 a.a. ~ 606 a.a) full-length human protein.
Sequence
MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
Host
Rabbit
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
ARAF MaxPab rabbit polyclonal antibody. Western Blot analysis of ARAF expression in human placenta.Western Blot (Transfected lysate)
Western Blot analysis of ARAF expression in transfected 293T cell line (H00000369-T03) by ARAF MaxPab polyclonal antibody.
Lane 1: ARAF transfected lysate(67.60 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with anti-ARAF rabbit purified polyclonal 1:1200 and anti-BRAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of purified MaxPab antibody to ARAF on HeLa cell. [antibody concentration 30 ug/ml] -
Gene Info — ARAF
Entrez GeneID
369GeneBank Accession#
NM_001654.1Protein Accession#
NP_001645.1Gene Name
ARAF
Gene Alias
A-RAF, ARAF1, PKS2, RAFA1
Gene Description
v-raf murine sarcoma 3611 viral oncogene homolog
Omim ID
311010Gene Ontology
HyperlinkOther Designations
OTTHUMP00000023212|Oncogene ARAF1|Ras-binding protein DA-Raf|v-raf murine sarcoma 3611 viral oncogene homolog 1
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Interactome
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Pathway
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