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BIOtech
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ANPEP 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000290-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ANPEP full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 109.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ANPEP antibody (H00000290-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000290-T02
    ANPEP transfected lysate.
    Western Blot
    QC Testing of H00000290-T02
    Lane 1: ANPEP transfected lysate ( 109.50 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 290
  • Gene Name:
  • ANPEP
  • Gene Alias:
  • APN,CD13,LAP1,PEPN,gp150,p150
  • Gene Description:
  • alanyl (membrane) aminopeptidase
  • Gene Summary:
  • Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000194690,aminopeptidase M,aminopeptidase N,membrane alanine aminopeptidase,microsomal aminopeptidase
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