ACADM 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACADM transfected lysate.
Western Blot
Lane 1: ACADM transfected lysate ( 46.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACADM full-length
Host
Human
Theoretical MW (kDa)
46.6
Interspecies Antigen Sequence
Mouse (87); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACADM antibody (H00000034-D01P) by Western Blots.
SDS-PAGE Gel
ACADM transfected lysate.
Western Blot
Lane 1: ACADM transfected lysate ( 46.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACADM
Entrez GeneID
34GeneBank Accession#
NM_000016.2Protein Accession#
NP_000007.1Gene Name
ACADM
Gene Alias
ACAD1, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH
Gene Description
acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Gene Ontology
HyperlinkGene Summary
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000011345|medium-chain specific acyl-CoA dehydrogenase
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Interactome
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Pathway
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Disease
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