The below solutions are our recommended steps to try when Trouble-shooting any products related issue. All of these steps are a great way to begin Trouble-shooting and/or to try when other solutions have not resolved your issue. When trying these steps, most users will either have their issues resolved.
If the Trouble-shooting tips are not able to solve your problems, please fill out Contact Us form , Abnova customer service staff will reply your request one to one.
Non-fat milk may mask antigens. Try to reduce the amount of milk in the blocking buffer and antibody solution or substitute it with different blocking reagents.
Use a secondary antibody which was rasied againt the host species of the primary antibody. Use positive controls to ensure that the secondary antibody is working properly.
Use higher concentrations of the antibody. Our protocol provides a guideline for antibody dilutions. Use positive controls to ensure that the primary antibody is working properly.
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