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SAMx™ Immune

SAMx™ Immune is a specialized self-amplifying mRNA (SAM) immunization platform providing the competitive attributes of convenience, flexibility, and functionality for antibody production.

For convenience, the target can be a cytoplasmic, membrane, or secretory full-length or partial gene of interest. After target gene cloning into an immunologically-driven vector, the proprietary plasmid is scaled-up, transcribed into mRNA by in vitro transcription (IVT), capped to prevent from nuclease digestion, digested of all DNA remaining products, and purified to replete all contaminants. SAM immunization obviates the need for protein production for immunization. Nonetheless, the SAM containing target-of-interest can be directly used for recombinant protein and lysate production for downstream antibody testing.

For flexibility, diverse mammalian species such as mouse, rat, and rabbit can be used for immunization to generate high-quality polyclonal and/or monoclonal antibodies. Moreover, monoclonality is achieved either by splenic myeloma fusion in mouse or rat, and by peripheral circulating B cell isolation and cloning in rabbit. Heavy and light chain in the variable domains can be bioengineered to create a variety of antibody constructs.

For functionality, high sensitivity and specificity are prerequisite for a successful antibody. Downstream validation assays such as IF, IHC, FC, antibody pair, and sandwich ELISA are setup upstream during the monoclonal screening to ensure the best functional clones are identified with the intended applications. If you have a need for a functional antibody, we have the experience, infrastructure, and human resource to achieve your antibody goal.

 
SAM Immunization Workflow

 
Advantages

  • Obviate protein antigen for immunization & screening
  • Suitable for membrane protein & difficult antigen
  • Simple antigen sequence manipulation & mutagenesis
  • Rapid turn-around time for polyclonal and monoclonal antibodies
  • Functional antibody recognizing native antigen
 
Comparison Between mRNA and DNA Immunization

  mRNA Immunization DNA Immunization
Construction Rapid, Scalable, Cost-effective Rapid, Scalable, Cost-effective
 Cell Culture Production or Fermentation Not required Not required
Non-Infectious Yes Yes
Risk of Integration into Host Genome No Yes
Membrane Penetration Cytoplasm Cytoplasm & Nucleus
Dose µg (1000x lower) mg
 
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