Immunoprecipitation (IP) uses antigen-antibody reaction principle to isolate and concentrate a specific protein from a mixture of proteins. For example, such process can be used to pull out the protein of interest from a cell extract or culture supernatant via a protein A/G-coupled agarose beads. This technique can be done manually or by a high throughput automated magnetic bead platform, Precipitor™ developed by Abnova.
Types of Immunoprecipitation:
Individual protein Immunoprecipitation (IP):
A method to isolate the protein of interest from the mixture of proteins.
Protein complex Immunoprecipitation (Co-IP):
A method to analyze protein-protein interactions. Co-precipitated protein can then be identified by western blot analysis or by sequencing a purified protein band.
Chromatin Immunoprecipitation (ChIP):
A method to determine the location of DNA binding sites on the genome for the protein of interest. This technique gives a picture of the protein-DNA interactions that occur inside the nucleus of living cells or tissues.
RNA Immunoprecipitation (RIP):
Similar to chromatin immunoprecipitation (ChIP), but rather than targeting DNA binding proteins as in ChIP, RNA immunoprecipitation targets RNA binding proteins.
Abnova's Precipitor™ system is automated magnetic bead platform for high throughput precipitation and purification of proteins. Combining 96 deep well plate with affinity conjugated magnetic beads, Precipitor™ easily handles 16 different assays simultaneously by transferring beads from one well to the next for mixing, binding, washing, and elution reactions via the robotic action of parallel magnetic rods. It simplifies the routine yet labor intensive process, and addresses the needs of rigorous proteomic screening and biomarker discovery applications such as immunoprecipitaton (IP, ChIP, RIP), recombinant protein purification, and protein-protein interaction. Precipitor™ delivers reproducible and consistent results by obviating the drawbacks of manual operation. Its integrated onscreen display allows easy change of parameters tailored to your experiment. Moreover, you can select from large scope of available antibodies reagents to accelerate your research!
Precipitor™ system, an automated magnetic bead platform for high throughput precipitation and purification of proteins. It easily handles 16 different assays simultaneously and addresses the needs of rigorous proteomic screening and biomarker discovery applications such as immunoprecipitaton (IP, ChIP, RIP), recombinant protein purification, and protein-protein interaction.
Precipitor™ system, an automated magnetic bead platform for high throughput precipitation and purification of proteins. We show you how to install Precipitor™ software, AutoMag, and how to modify the parameters easily.
Precipitor™ system, an automated magnetic bead platform for high throughput precipitation and purification of proteins. We show you how to operate Precipitor™ and display each accessory. Watch this video before turning on the power.
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