Details of the payment will be advised on the invoice that will besent together with your shipment.
The following methods of payment are accepted:
• Credit card
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We do not accept money orders or cash.
If you find any defect upon receipt of the product(s), please inform us within 10 days. We will replace the faulty product(s) immediately.
If you received a product that does not work in your experiment, you do not need to return it; however, we do need your experimental data for the next process. Please obtain a troubleshooting form from us to fill in; we will make sure that you receive full support from our professional team.
If the antigen is a peptide, 2 mg will usually be sufficient before conjugation (1 mg for KLH, and 1 mg for BSA). If the antigen is a protein, 500µg/mice at concentration of 0.5-1 mg/ml is usually sufficient.
A novel highly efficient and robust wheat germ cell-free protein synthesis
system was developed by Professor Yaeta Endo of Ehime University, Japan.
The RNA N-glycosidase tritin and other inhibitors such as thionin,
ribonucleases, deoxyribonucleases, and proteases that originate from the
endosperm and inhibit translation were removed by extensive washing of
wheat germ embryo. The in vitro expression using the extract from wheat germ devoid of the inhibitors and optimized expression vector is highly efficient and stable, and is capable of translating a full length protein as long as 70-80 kDa.
Publications related to Wheat Germ in vitro expression system are listed
below:
1: Sawasaki T, Ogasawara T, Morishita R, Endo Y. A cell-free protein synthesis system for high-throughput proteomics.
Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14652-7. Epub 2002 Oct 30.
PMID: 12409616 [PubMed - indexed for MEDLINE]
2: Sawasaki T, Hasegawa Y, Tsuchimochi M, Kamura N, Ogasawara T, Kuroita T, Endo Y. A bilayer cell-free protein synthesis system for high-throughput screening of gene products.
FEBS Lett. 2002 Mar 6;514(1):102-5.
PMID: 11904190 [PubMed - indexed for MEDLINE]
3: Morita EH, Sawasaki T, Tanaka R, Endo Y, Kohno T. A wheat germ cell-free system is a novel way to screen protein folding and function.
Protein Sci. 2003 Jun;12(6):1216-21.
PMID: 12761392 [PubMed - in process]
4: Kigawa T, Yabuki T, Yoshida Y, Tsutsui M, Ito Y, Shibata T, Yokoyama S. Cell-free production and stable-isotope labeling of milligram quantities of proteins.
FEBS Lett. 1999 Jan 8;442(1):15-9.
PMID: 9923595 [PubMed - indexed for MEDLINE]
5: Madin K, Sawasaki T, Ogasawara T, Endo Y. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes.
Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):559-64.
PMID: 10639118 [PubMed - indexed for MEDLINE]
We usually obtain 5ml of ascites from each Balb/c mouse and 8-10ml from each SCID mouse. The custom ascites service is based on number of mice used to generate the quantity of ascites needed by the customer. However, we cannot guarantee the same yield for every cell line since each may have a distinct growth characteristic. However, if an absolute quantity is desired, we can fulfill the request by inject more mice.
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