Chimera RNA interference (chimera RNAi) is a process by which small interfering RNA/DNA chimera triggers
the destruction of mRNA. The discovery work, design, and application of chimera RNAi have been pioneered by Professor
Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.
Chimera RNAi has many advantages over the conventional siRNAs. First, it has been demonstrated to have
reliable knock-down for over 10,000 human genes. Because the human genome is composed of an intricate, genetic network,
chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence. Another
advantage of the chimera RNAi technology is its effectiveness
at low concentrations (0.5nM to 5nM); only mRNA is destroyed
so genomic genes are not affected. Finally, having both the
sense and anti-sense strands consisting RNA/DNA chimera, it
offers much greater compound stability for streamlining in
vitro and in vivo assays and applications while minimizing
interferon induction and other adverse reactions.