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Orthotopic Xenograft Tumor Models

Various mouse models have been developed and widely exploited in cancer research. To evaluate the efficacy of cancer treatments in preclinical and clinical studies, human tumor xenograft models whereby human cell lines are transplanted into immunocompromized mice are commonly used. In particular, orthotopically implantation of human tumor cells into the equivalent mouse organ where the cancer originated is preferred for demonstrating the effects of new cancer drugs on specific tumors because it provides tumor cells a microenvironment for organotypic interaction which may affect tumor growth, differentiation and its response to drugs. Orthotopic tumor models offer more clinical relevance for tissue site-specific pathology and hence provide prospective evaluation for chemotherapeutic drugs.

With superior skills, Abnova has successfully established several orthotopic human tumor models for researchers to evaluate in vivo efficacy of new compounds for cancer therapy, and more orthotopic models for 8 most common human cancers are in development. With a luciferase expressing cell line directly injected into relevant organ of a live mouse, the orthotopically implanted tumor cells can be easily detected and the growth of tumor in response to anticancer compounds can be monitored in vivo with the IVIS® biophostonic imaging system. Besides providing orthotopic mouse models for different cancers, we also offer customized drug injection service for the convenience of our customers.
 
Advantages

  • Increased clinical relevance compared to subcutaneous xenografts
  • Reconstitution of relevant tumor microenvironment
  • Assess of tumor-stromal interactions
  • Site specific study for anti-tumor treatment
  • Use of modeling clinical course of metastatic diseases
 
Our Services

  • Orthotopic implantation of tumor cells with flawless surgical techniques
  • 8 major tumor types (lung, breast, colon, prostate, liver, pancreas, ovary and spleen)
  • Evaluation of tumor growth in orthotopic xenograft model in vivo
  • Drug treatment with customized methods: s.c, i.p, or i.v.
  • Stable cell lines provided for standard orthotopic efficacy study
  • Our luciferase-labelled tumor cell line available for animal model pre-test/experiment
 
Developed Orthotopic Mouse Models for Human Cell Lines

Human Cancer Tumor Cell Line Mouse Strain Mouse Organ
Hepatocellular carcinoma HepG2 NOD/SCID Liver
Lung carcinoma A549 NOD/SCID Lung
Lung carcinoma CL1-5 NOD/SCID Lung
Lung carcinoma CL1-5 Nude Lung
*More models are in development and the list is increasing.
 
Examples

Luciferase expression from orthotopic liver tumor in anesthetized NOD/SCID mice
(stable cell line used: HepG2-Luc)
 
Luciferase expression from orthotopic lung tumor in anesthetized NOD/SCID mice
(stable cell line used: A549-Luc)
 

For any inquiry, please contact : OEM@abnova.com

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