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Circulating Tumor DNA

Abnova provides an ISO15189 medical laboratory service to physicians and clinical researchers investigating circulating tumor DNA (ctDNA) released into the peripheral blood from tumor mass of cancer patients. Our capability is supported by a streamlined platform for ctDNA extraction, purification, library preparation and next-generation sequencing. ctDNA liquid biopsy is a precision tool for identifying and tracking cancer transformation and metastasis. It has significant clinical impact in cancer diagnosis, prognosis, treatment, and monitoring in the new era of personalized medicine.
 
Flowchart

 
Specimen Requirement

  • Human whole blood: collect at least 4ml whole blood in EDTA tube (purple cap) at room temperature.
  • Sample shipment: keep whole blood sample in room temperature after blood collection and throughout transportation.
  • DNA preparation: extract genomic DNA from the plasma fraction of the whole blood sample on the same day of collection and store at 4°C.
 
Whole Blood Fractionation, Genomic DNA Extraction, Library Preparation, Next-Generation Sequencing, and Gene Mutation Analysis

  • Whole blood fractionation: centrifuge and separate the whole blood into plasma, buffy coat (white blood cells & platelets), and erythrocyte fractions.
  • DNA extraction: extract genomic DNA from the plasma fraction followed by DNA purification and quantification.
  • Library preparation: fragment and size the genomic DNA, convert to double-stranded DNA, attach oligonucleotide adaptors, and quantitate library product.
  • Next-generation sequencing: perform hotspot and exon sequencing of mutated genes of interest.
  • Gene mutation analysis: annotate and compare cancer gene mutations including variants with published literature and public databases.
 
Analytical Methods

  • Next Generation Sequencing (NGS)
 
Deliverables (Sent within 14 Working Days)

An interpretative report of the cancer gene mutations will be provided.
  • Targeted genes used to identify cancer mutations.
  • Sequences of cancer gene mutations and their variants based on next-generation sequencing.
  • Cancer mutation annotation and comparison with known datasets.
  • Additional mutations gene sequencing per customer’s request.
 
Services

Cancer Panels Cancer Genes & Variants Service Fee
Actionable Insights Tumor Panel V2 ALK
BRAF
EGFR
ERBB2
ERBB3
ESR1
KIT
KRAS
NRAS
PDGFRA
PIK3CA
RAF1
Inquire
Actionable Insights Tumor Panel V3 AKT1
ALK
BRAF
CTNNB1
DDR2
EGFR
ERBB2
ERBB3
ERBB4
ESR1
FBXW7
FGFR1
FGFR2
FGFR3
FLT3
GNA11
GNAQ
HRAS
KIT
KRAS
MAP2K1
MAP2K2
MET
NOTCH1
NRAS
PDGFRA
PIK3CA
RAF1
SMAD4
STK11
Inquire
Customized Tumor Panel Upon Request Inquire
 
 

For any inquiry, please contact : OEM@abnova.com

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