Abnova’s DNAx™ Immune is a specialized DNA immunization platform with competitive attributes of convenience, flexibility, and functionality for antibody production. We provide diverse mammalian species such as mouse, rat, and rabbit for immunization to generate high-quality polyclonal and monoclonal antibodies based on customers’ gene of interest. Different assays such as IF, IHC, FC, antibody pair, and sandwich ELISA are setup for antibody validation to ensure the best functional antibodies are identified with the intended applications. If you have a need for a functional antibody, we have the experience, infrastructure, and human resource to achieve your antibody goal.
See Technology
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| Advantages |
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- Obviate protein antigen for immunization & screening
- Suitable for membrane protein & difficult antigen
- Simple antigen sequence manipulation & mutagenesis
- Rapid turn-around time for polyclonal and monoclonal antibodies
- Functional antibody recognizing native antigen
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| DNAx™ Immune Service Summary |
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Services Items |
Lead Time |
| Phase I |
Target-of-Interest |
Customer-supplied target gene:
- Cytoplasmic, membrane, secretory
- Full-length, partial
Please quote if the customer requires gene prepared by Abnova |
3 weeks |
| Gene Cloning |
Vector gene target cloning |
| Plasmid Preparation |
Extraction and purification for plasmid DNA from E. coli |
2 weeks |
| Intra-muscular Immunization |
Number of mice, rats or rabbits immunized will be specified by customers |
45 days |
| Polyclonal Sera Screening |
Plasmids are transferred to 293T cells to generate overexpression lysate and screen target DNA |
2 weeks |
| Phase II |
Polyclonal Antibody |
Purification |
Protein A purification |
2 weeks |
| Validation |
Antibody validation using WB, IHC, FC, Ab Pair or Sandwich ELISA per customers' request |
| Final Deliverables |
Desired polyclonal antibody |
| Monoclonal Antibody |
Production |
Splenic myeloma fusion in mouse or rat
Peripheral circulating B cell isolation and cloning in rabbit |
12-16 weeks |
| Validation |
Antibody validation using WB, IHC, FC, Ab Pair or Sandwich ELISA per customers' request |
| Final Deliverables |
Desired monoclonal, supernatant and hybridoma |
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For any inquiry, please contact : OEM@abnova.com
