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Custom Rabbit Monoclonal Antibody Production

Abnova recognizes the importance of rabbit monoclonal antibody for research, development and commercialization. Technology accessibility, intellectual property, and higher cost have long hampered the widespread adoption and application of rabbit monoclonal antibody. Unlocking this barrier is critical to success of proteomics and biotech industry where traditional antibody tools such as mouse monoclonal antibody and rabbit polyclonal antibody encounter their limitations.

Abnova Rabbit Monoclonal (ARM) technology is an integrated and high throughput platform for the generation of rabbit monoclonal antibody. This service is available to everyone in the research and in vitro diagnostic community. A wide variety of antigens can be used for rabbit immunization. Rabbit possesses a broader yet non-tolerant immunologic repertoire for antibody development. A non-fusion library is created to screen for antibody clone of highest affinity and exquisite specificity. The clone is introduced into a proprietary expression vector for transient and scalable antibody production for many downstream applications. Customers can access the rabbit serum for pre-testing, antibody sequencing for antibody engineering, and purified antibody for application of interest.
 
Related FAQ
Custom Rabbit Monoclonal Antibody Development Workflow

  • Evaluation of Standard Antibody Production Service
  • Pre-Production Discussion: Custom Specification, Flowchart, Cost
  • Purchase Order
  • Production Specification Approval
  • Service Agreement and Production Specification Template
  • Monoclonal Antibody Development and Progress Report
  • Purified Monoclonal Antibodies Delivery
 
 
 
ARM vs. Conventional Rabbit Mab

  Abnova Rabbit Mab (ARM) Conventional Rabbit Mab
 Technology Non-Fusion Fab Antibody Library Rabbit Myeloma
 Antibody Repertoire Broad (no fusion needed) Narrow (<1% fusion rate)
 Screening Throughput High Low
 Epitope Coverage High Low
 Antibody Success Rate Higher Lower
 Antibody Yield High Low
 Clone Stability Excellent Poor
 Antibody Engineering Readily Applicable Troublesome
 
 
Rabbit vs. Mouse Mab

  Rabbit Mouse
Immune Tolerance None Yes
Antibody Diversity Broad Narrow
Antigenicity Strong reaction to small molecules,peptides, & proteins. Weak reaction to small molecules and peptides.
Affinity Picomolar Nanomolar
Specificity High Moderate
Isotype IgG IgG1, IgG2a, IgG2b, IgG3
Applications Especially suited for IHC, IP, FC, antibody pair & kit. Mostly suited for WB, ELISA, IF, antibody pair & kit.
Antibody Engineering Yes Yes
 
 
Advantages Applications

  • High Affinity Antibody
  • Diverse Antibody Repertoire
  • No Immunologic Tolerance
  • Scalable Antibody Production
  • More Stable Antibody Clone
  • Antibody Engineering
  • High Affinity Antibody
  • Rabbit Monoclonal Antibody
  • Modification-State Antibody
  • Anti-Idiotype Antibody
  • Antibody Pairing and ELISA Kit
  • Research and In Vitro Diagnostic
 
 
Demonstrations

Comparison of Anti-TSH Rabbit Mab With a Commercial Antibody

TSH Dose Response Curves
Comparison of isolated rabbit Mab for TSH (thyroid stimulating hormone) with a commercial antibody diagnostic kit on detection of TSH in a sandwich ELISA setting. The rabbit Mab shows a superior binding activity to TSH compared side-by-side to the commercial antibody.

 
 
Validation of Isolated Anti-Collagen Rabbit Mab by Sandwich ELISA

 

NTx Standard Curve
A rabbit Mab was used to capture a collagen peptide (NTx) with serial dilutions (0, 0.05, 0.10, 0.25, 0.50 and 1.00 ug/ml) in physiologic relevant concentration range in a sandwich ELISA setting.

 

For any inquiry, please contact : OEM@abnova.com

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