Recombinant Protein

Abnova offers high quality, efficient and cost-saving conjugation service between protein and fluorescent dyes, phycobiliproteins, streptavidin, biotin and enzymes. Since every protein has its own unique characteristics, conjugation needs to be optimized. 0.2mg of total protein of interest will be tested and analyzed prior to mass labeling. The tested sample will be mixed with mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the protein sample.

General Working Progress of Protein Conjugation

Download Custom Recombinant Protein Conjugation

Evaluation and Discussion of Conjugation Requirement and Service
Completion of the Custom Conjugation Specification (,)
Confirmation of Receipt of Antibody/Protein Sample
Conjugation Buffer Exchange
Antibody/Protein Concentration and Volume Analysis

Test-Scale Conjugation Optimization
Mass Antibody/Protein Labeling
Chromatographic Gel Purification
Spectrophotometer Measurement
COA, Packing, and Shipment

Table of Labeling Molecules and Excitation/Emission Wavelengths

Labeling Molecule	Excitation Max (nm)	Emission Max (nm)
Biotin	-	-
HRP	-	-
Alexa 488	490	525
FITC	490	525
PE	535	575
TRITC	546	575
Cy3	554	568
Alexa 555	555	580
Alexa 594	590	617
Texas Red	595	616
APC	650	660
Alexa 647	650	665
Cy5	650	670
DyLight 650	652	672

Special Notes:

The protein of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of protein from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer.
Minimum quanity of antibody conjugation is 0.2mg. Minimum concentration is 0.1mg/ml.

Example

QC Data of Fluorochrome-Labeled Protein

The PC9 were detected by FITC conjugated PanCK monoclonal antibody (green), white blood cells were detected by PE conjugated CD45 monoclonal antibody (orange) and nucleus were detected by DAPI counter stain (blue).

For any inquiry, please contact : OEM@abnova.com