Abnova recognizes the importance of rabbit monoclonal antibody for research, development and commercialization. Technology accessibility, intellectual property, and higher cost have long hampered the widespread adoption and application of rabbit monoclonal antibody. Unlocking this barrier is critical to success of proteomics and biotech industry where traditional antibody tools such as mouse monoclonal antibody and rabbit polyclonal antibody encounter their limitations.

Abnova Rabbit Monoclonal (ARM) technology is an integrated and high throughput platform for the generation of rabbit monoclonal antibody. This service is available to everyone in the research and in vitro diagnostic community. A wide variety of antigens can be used for rabbit immunization. Rabbit possesses a broader yet non-tolerant immunologic repertoire for antibody development. A non-fusion library is created to screen for antibody clone of highest affinity and exquisite specificity. The clone is introduced into a proprietary expression vector for transient and scalable antibody production for many downstream applications. Customers can access the rabbit serum for pre-testing, antibody sequencing for antibody engineering, and purified antibody for application of interest.

Related FAQ

Custom Rabbit Monoclonal Antibody Development Workflow

  • Evaluation of Standard Antibody Production Service
  • Pre-Production Discussion: Custom Specification, Flowchart, Cost
  • Purchase Order
  • Production Specification Approval
  • Service Agreement and Production Specification Template
  • Monoclonal Antibody Development and Progress Report
  • Purified Monoclonal Antibodies Delivery
 
 
 

 

ARM vs. Conventional Rabbit Mab

 

Abnova Rabbit Mab (ARM)

Conventional Rabbit Mab

Technology

Non-Fusion Fab Antibody Library

Rabbit Myeloma

Antibody Repertoire

Broad (no fusion needed)

Narrow (<1% fusion rate)

Screening Throughput

High

Low

Epitope Coverage

High

Low

Antibody Success Rate

Higher

Lower

Antibody Yield

High Low

Clone Stability

Excellent Poor

Antibody Engineering

Readily Applicable Troublesome
 

 

Rabbit vs. Mouse Mab

 

Rabbit

Mouse

Immune Tolerance

None

Yes

Antibody Diversity

Broad

Narrow

Antigenicity

Strong reaction to small molecules,peptides, & proteins.

Weak reaction to small molecules and peptides.

Affinity

Picomolar

Nanomolar

Specificity

High

Moderate

Isotype

IgG IgG1, IgG2a, IgG2b, IgG3

Applications

Especially suited for IHC, IP, FC, antibody pair & kit. Mostly suited for WB, ELISA, IF, antibody pair & kit.

Antibody Engineering

Yes Yes
 


Advantages

  • High Affinity Antibody
  • Diverse Antibody Repertoire
  • No Immunologic Tolerance
  • Scalable Antibody Production
  • More Stable Antibody Clone
  • Antibody Engineering

Application

  • High efficiency
  • High Affinity Antibody
  • Rabbit Monoclonal Antibody
  • Modification-State Antibody
  • Anti-Idiotype Antibody
  • Antibody Pairing and ELISA Kit
  • Research and In Vitro Diagnostic
   

 

Demonstrations

Comparison of Anti-TSH Rabbit Mab With a Commercial Antibody

 

rabbit_S-02.jpg (86 KB)

TSH Dose Response Curves

Comparison of isolated rabbit Mab for TSH (thyroid stimulating hormone) with a commercial antibody diagnostic kit on detection of TSH in a sandwich ELISA setting. The rabbit Mab shows a superior binding activity to TSH compared side-by-side to the commercial antibody.

 

Validation of Isolated Anti-Collagen Rabbit Mab by Sandwich ELISA

 

rabbit_S-03.jpg (84 KB)

NTx Standard Curve

A rabbit Mab was used to capture a collagen peptide (NTx) with serial dilutions (0, 0.05, 0.10, 0.25, 0.50 and 1.00 ug/ml) in physiologic relevant concentration range in a sandwich ELISA setting.

For any inquiry, please contact : OEM@abnova.com