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Custom Polyclonal Antibody Production

Abnova has extensive experience in generating high quality antisera to a wide variety of antigens (peptides, phospho-peptides, proteins, and cDNAs). Using a low dose immunization technique in combination with a proprietary adjuvant, we are able to generate a high-titer, high-affinity antibody response against the immunogen in mice, rats, and rabbits.
 
Polyclonal Antibody Development Workflow

  • Evaluation of Standard Antibody Production Service
  • Pre-Production Discussion: Custom Specification, Flowchart, Cost
  • Purchase Order
  • Production Specification Approval
  • Production Specification Template
  • Polyclonal Antibody Development and Progress Report
  • Polysera Delivery
 
 Mouse & Rat
 Service Items Time
(weeks)
Antigen Supplied by Customer Antigen Supplied by Abnova
Peptide Phospho-peptide Protein cDNA
Peptide Design & Synthesis
Customer-supplied antigen 4 ~ 5 V V V              
7~20-mer (Purity > 80%)       V V V        
Single site phosphorylation
(Ser, Tyr, Thr)
          V        
Conjugation
To carrier protein KLH/BSA/OVA
(A cysteine is automatically added to the C-terminus or N-terminus of the peptide)
1       V   V        
Protein Preparation
cDNA sourcing 2~3             V V    
Cloning and sequencing
Protein expression
Immunization
5~8 injections per animal
(3 mice or 3rats)
8 ~ 12 V V V V V V V V V V
Sera collection
Report
ELISA data * 1 V V V V V V V V V V
WB data (mammalian overexpression lysate)                 V V
Purification **
By protein A purification
Final deliverable: > 0.2 mg purified antibody for mice or > 5 mg purified antibody for rats
2   V   V     V   V  
By affinity purification
(for rats only)
Final deliverable: > 0.5 mg purified antibody
    V   V V   V   V
Total Lead time (weeks) 10~12 12~14 12~14 17~20 17~20 17~20 19~20 19~20 12~16 12~16
Total Price (USD/EUR) $750 $1,100 $1,500 Quote Quote Quote Quote Quote Quote Quote
 
 Rabbit  
 Service Items Time
(weeks)
Antigen Supplied by Customer Antigen Supplied by Abnova
Peptide Phospho-peptide Protein cDNA
Peptide Design & Synthesis
Customer-supplied antigen   4 ~ 5 V V V              

7~20-mer (Purity > 80%)

      V V V        
Single site phosphorylation
(Ser, Tyr, Thr)
          V        
Conjugation
To carrier protein KLH/BSA/OVA
(A cysteine is automatically added to the C-terminus or N-terminus of the peptide)
1       V V V        
Protein Preparation
cDNA sourcing 2~3             V V    
Cloning and sequencing
Protein expression
Immunization
5~8 injections per rabbit
(2 rabbits)
10 ~ 12 V V V V V V V V V V
Sera collection
Report
ELISA data * 1 V V V V V V V V V V
WB data (mammalian overexpression lysate)                 V V
Purification **
By protein A purification
Final deliverable: 20-50 mg purified antibody
2   V   V     V   V  
By affinity purification
Final deliverable: 1-5 mg purified antibody
    V   V V   V   V
Total Lead time (weeks) 11~14 13~16 13~16 17~20 17~20 17~20 19~20 19~20 12~16 12~16
Total Price (USD/EUR) $750 $1,300 $1,600 Quote Quote Quote Quote Quote Quote Quote
Note:
* Polyclonal antibody sera will be QC utilizing ELISA format for reactivity against the immunogen.
** This is the average yield. Actual yield will vary for each Pab.
The above list price is only used for direct orders made to Abnova.
 
 
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