Products

Description

The RNA Marker Low consists of seven single-stranded RNAs. The 20-base and 50-base RNA are synthesized by chemically (not phosphorylated). The 100, 200, 300, 400 and 500 bases are synthesized by in vitro transcription. The RNA Marker Low is suitable for determinating size of single-stranded RNAs in denaturing polyacrylamide gel electrophoresis. The concentration of each RNA (20-500 base) in the marker is approximately 0.1 ug/ul. It is useful for estimating of RNA amount. The RNA Marker Low can be visualized by ethidium bromide staining.

Description

The RNA Marker Low Easy is supplied in a ready-to-use mixture of loading dye (containing formamide, EDTA sodium salt, bromphenol blue) and RNAs. It is prepared for denaturing polyacrylamide gel electrophoresis but not agarose gel elctrophoresis. The RNA Marker Low Easy has seven single-stranded RNAs, 20, 50, 100, 200, 300, 400 and 500 bases. The 20-base and 50-base RNA are synthesized by chemically (not phosphorylated), others are synthesized by in vitro transcription. In 5 ul of the RNA Marker Low Easy, each RNA amount is approximately 100 ng. It is useful for estimating RNA amount approximately. The RNA Marker Low Easy can be visualized by UV light after ethidium bromide staining.

Description

The RNA Marker High consists of nine single-stranded RNAs, 200, 500, 1,000, 1,500, 2,000, 3,000, 4,000, 5,000 and 8,000 bases, which are synthesized by in vitro transcription. The RNA Marker High is suitable for determinating size of single-stranded RNAs in denaturing agarose gel electrophoresis. The concentration of each RNA (200-8,000 base) in the marker is approximately 0.1 ug/ul. It is useful for estimating of RNA amount. The RNA Marker High can be visualized by UV light after ethidium bromide staining or exposure to film with end labeling.

Description

This product is manufactured for easy measurement of RNA size on agarose gel electrophoresis. With this product, electrophoresis is possible on non-denaturing agarose gel as well as on denaturing agarose gel. To use this product, just mix RNA loading buffer AG with formaldehyde solution (not included in this product), and added the mixture to RNA Marker High Easy or RNA sample, then heat to load into wells of agarose gel. The RNA loading buffer AG+ is a gel loading buffer prepared not only for denaturing agarose gel electrophoresis but also for non-contained denaturing agarose gel electrophoresis. The RNA Marker High Easy contains nine single-stranded RNAs, 500, 1,000, 1,500, 2,000, 3,000, 4,000, 5,000 and 8,000 bases. Each RNA is 0.1 ug/uL approximately. It is useful for estimating RNA amount approximately. After electrophoresis, RNA bands on agarose gel can be visualized by UV light without further ethidium bromide staining.

Description

The dsRNA Marker consists of ten double-stranded RNAs, 10, 20, 30, 50, 100, 200, 300, 400, 500 and 1,000 base pairs. The dsRNA Marker is an ideal size marker for determinating sizes of double-stranded RNAs. A twenty bp of RNA band including in the dsRNA Marker is adjusted to approximately 25 ng/ul in concentration. The dsRNA Marker is manufactured for non-denaturing polyacrylamide gel electrophoresis. The dsRNA Marker can be visualized by UV light after ethidium bromide staining.

Description

The dsRNA Marker Easy is supplied in a ready-to-load mixture of loading dye (containing Tris-HCl buffer, glycerol, EDTA sodium salt, sodium chloride, bromphenol blue) and an ideal size marker for determinating sizes of double-stranded RNAs. The dsRNA Marker Easy consists of ten double-stranded RNAs, 10, 20, 30, 50, 100, 200, 300, 400, 500 and 1,000 base pairs. In 5 ul of the dsRNA Marker Easy, a 20 bp of dsRNA is approximately 50 ng. The dsRNA Marker Easy can be visualized by UV light after ethidium bromide staining.

Description

The Small RNA Marker consists of five single-stranded RNAs (ssRNA). The 20, 30, 40 and 50 bases RNAs are synthesized by chemically (non phosphorylated). The 100 bases RNA is synthesized by in vitro transcription. The Small RNA Marker is suitable for determinating size of small-size ssRNA in denaturing polyacrylamide gel electrophoresis. The Small RNA Marker can be visualized by ethidium bromide staining.

Description

The Small RNA Marker Easy has five single-stranded RNAs, 20, 30, 40, 50 and 100 bases, which is useful for a research of small RNAs. The 20, 30, 40 and 50 bases are synthesized by chemically (non phosphorylated). The 100 bases is synthesized by in vitro transcription. The Small RNA Marker Easy is supplied in a ready-to-use mixture of loading dye and RNAs (containing formamide, EDTA sodium salt, bromphenol blue). It is manufactured for denaturing polyacrylamide gel electrophoresis. The Small RNA Marker Easy can be visualized by UV light exposure after ethidium bromide staining.

Description

The Small RNA Prestain Marker Plus consists of six prestained single-strand (blue and red) nucleic acids (apparent molecular weights are 20, 30, 40, 50, 75 and 100 bases) and it is visible during electrophoresis. The Small RNA Prestain Marker Plus is suitable for monitoring denaturing polyacrylamide gel electrophoresis and blotting onto membranes. The apparent sizes of bands in Small RNA Prestain Marker Plus are in excellent agreement with sizes of non-stained RNAs, 20, 30, 40, 50, 75 and 100 bases in length (about 95% accuracy). The Small RNA Prestain Marker Plus is supplied in a ready-to-use mixture and doesn't require heating or addition of a denaturing agent before use.

Description

The RNA Prestain Marker High is a RNA ladder for precise sizing of RNA molecules in denaturing agarose gels. The RNA Prestain Marker High is a visible molecular weight marker for ssRNA, consisting of six colored (blue and purple) nucleic acids. The six colored bands (apparent molecular weights are 200, 500, 1,000, 2,000, 4,000 and 8,000 bases) are suitable for monitoring denaturing agarose gel electrophoresis and blotting onto membranes. The RNA Prestain Marker High shows the same mobility as that of the RNA Marker High (Cat # R0003) on denaturing agarose gel electrophoresis (>90% accuracy, see table 1). The RNA Prestain Marker High is supplied in a ready-to-use mixture without requiring heating or the addition of a denaturing agent before use.