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DEPC-treated Water is can be used in various applications in molecular biology, paticulary for use of RNA. The DEPC-treated Water was prepared by 0.1 % DEPC-treatment and autoclave of highly purified water. The product was confirmed to be RNase-free and DNase free. Apperance is clear liquid.
RNA Staining Solution (X 1) is developed to stain RNA in polyacrylamide gel and to excise RNA from the gel. The detection limit of RNA is as low as 50 ng. The sensitivity is approximately five times higher than that of UV shadowing. Stained RNA band excised from polyacrylamide gel can be extracted by a crush and soak method followed by ethanol precipitation. Obtained RNA can be used for RT-PCR, enzyme reaction and labeling reaction. RNA Staining Solution (X 1) does not require a UV transilluminator to detect RNA on polyacrylamide gel because bands are visible under daylight.
RNase-free Water is suitable to experiment and research of RNA. The RNase-free Water, non DEPC was prepared from highly purified water by ultrafiltration without DEPC treatment. The product was confirmed to be free from RNase and DNase. Apperance is clear liquid.
Salmon Sperm DNA was shared by sonication.DNA is dissolved in deionized distilled water, with a fragment size range of 300-700 base pairs.