RNase Inhibitor
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Specification
Product Description
Murine RNase inhibitor binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the enzymes and protecting RNA from degradation.
Host
Escherichia coli
Preparation Method
Escherichia coli expression system
Endotoxin Level
≤ 10 EU/mg
Activity
The amount of murine RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50% was defined as one unit (U)
Quality Control Testing
Exonuclease Activity: 40 U of murine RNaseinhibitor with 1 ug Lambda-Hind III digest DNA at 37°C for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Endonuclease Activity: 40 U of murine RNase inhibitor with 1 ug Lambda DNA at 37°C for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Nicking Activity: 40 U of murine RNase inhibitor with 1 ug pBR322 at 37°C for 16 hours yields no degradation as determined by agarose gel elStorage Buffer
In 50 mM KCl, 20 mM HEPES-KOH, pH 7.6 (8 mM DTT and 50% glycerol)
Storage Instruction
Store at -20°C.
Note
It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus.
Compared to human RNase inhibitors, murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation which causes inactivation of the inhibitor. That making it stable at low concentrations of DTT (less than 1 mM). -
Applications
Functional Study
In vitro Transcription
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