Fluorescent Dye Eu-Streptavidin
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Maximum Excitation/Emission wavelength: 346/617 nm
Maximum Excitation/Emission wavelength: 346/617 nm
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Specification
Product Description
Fluorescent Dye Eu with Streptavidin conjugate. Fluorescent Dye Eu is optimized for TR-FRET applications with large Stokes shifts and extremely long emission half-lives when compared to traditional fluorophores such as Alexa Fluor® or Cyanine dyes.
Typical Acceptor for TR-FRET
Alexa 647, APC, Cy5, DyLight 650, Fluorescence Dye 647-I, TF5
Molar Extinction Coefficient
>22,000M-1cm-1
Excitation (Max)
346nm
Emission (Max)
617nm
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 346/617 nm
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Applications
Conjugation
Time Resolved-Fluorescence Resonance Energy Transfer
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