Fluorescent Dye 780-M Streptavidin
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Maximum Excitation/Emission wavelength: 629/780 nm
Maximum Excitation/Emission wavelength: 629/780 nm
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Specification
Product Description
Fluorescence Dye 780-M with Streptavidin conjugate. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
Spectral Equivalent
APC-Alexa Fluor 750 Tandems
Molar Extinction Coefficient
≥80,000M-1cm-1
Excitation (Max)
629nm
Emission (Max)
780nm
Recommended Excitation
Red (633nm)
Recommended Filter Sets
Cy7 Filter
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 629/780 nm
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Applications
Conjugation
Flow Cytometry
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