Fluorescence Dye 750-I with Streptavidin conjugate. Compared with Alexa Fluor 750, Fluorescent Dye 750-I is much brighter with absorption at 633nm. Fluorescence emission of Fluorescence Dye 750-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Fluorescent Dye 647-I and R-phycoerythrin, making it ideal for multi-color labeling.
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 g/mL is sufficient. The optimal working dilution should be determined by the end user.
Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
Maximum Excitation/Emission wavelength: 749/775 nm