Fluorescent Dye 750-I Streptavidin
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Maximum Excitation/Emission wavelength: 749/775 nm
Maximum Excitation/Emission wavelength: 749/775 nm
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Specification
Product Description
Fluorescence Dye 750-I with Streptavidin conjugate. Compared with Alexa Fluor 750, Fluorescent Dye 750-I is much brighter with absorption at 633nm. Fluorescence emission of Fluorescence Dye 750-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Fluorescent Dye 647-I and R-phycoerythrin, making it ideal for multi-color labeling.
Spectral Equivalent
Alexa Fluor 750, Cy7, DyLight 755
Molar Extinction Coefficient
≥240,000M-1cm-1
Excitation (Max)
749nm
Emission (Max)
775nm
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 749/775 nm
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Applications
Conjugation
Flow Cytometry
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