Fluorescent Dye 647-I Streptavidin
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Specification
Product Description
Fluorescence Dye 647-I with Streptavidin conjugate. The slight red shift in absorption spectrum makes Fluorescence Dye 647-I an optimal match to filters designed Cy5 dyes.Besides, their fluorescence is pH-independent from pH 3 to 11.
Spectral Equivalent
Alexa Fluor 647, Cy5, DyLight 650
Molar Extinction Coefficient
≥ 240,000M-1cm-1
Excitation (Max)
649nm
Emission (Max)
664nm
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 649/664 nm
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Applications
Conjugation
Flow Cytometry
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