Fluorescent Dye 633-I Streptavidin
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Specification
Product Description
Fluorescence Dye 633-I with Streptavidin conjugate. Fluorescence emission of Fluorescence Dye 633-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Texas Red, Alexa Fluor 594, Fluorescent Dye 594-I and R-phycoerythrin, making it ideal for multi-color labeling. In comparison with Alexa Fluor 633, the extinction coefficient of Fluorescence Dye 633-I is much higher (250,000 cm-1M-1).
Spectral Equivalent
Alexa Fluor 633, DyLight 633
Molar Extinction Coefficient
> 240,000M-1cm-1
Excitation (Max)
638nm
Emission (Max)
655nm
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 638/655 nm
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Applications
Conjugation
Flow Cytometry
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Publication Reference
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Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis.
Rosemarie Ungricht, Laure Guibbal, Marie-Christine Lasbennes, Vanessa Orsini, Martin Beibel, Annick Waldt, Rachel Cuttat, Walter Carbone, Anne Basler, Guglielmo Roma, Florian Nigsch, Jan S Tchorz, Dominic Hoepfner, Philipp S Hoppe.
Cell Stem Cell 2022 Jan; 29(1):160.
Application:IF, Human, Kidney organoids (AG09429 cells).
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Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis.
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