Fluorescence Dye 633-I with Streptavidin conjugate. Fluorescence emission of Fluorescence Dye 633-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Texas Red, Alexa Fluor 594, Fluorescent Dye 594-I and R-phycoerythrin, making it ideal for multi-color labeling. In comparison with Alexa Fluor 633, the extinction coefficient of Fluorescence Dye 633-I is much higher (250,000 cm-1M-1).
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient. The optimal working dilution should be determined by the end user.
Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
Maximum Excitation/Emission wavelength: 638/655 nm