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Last updated: 2022/6/26

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Fluorescent Dye 594-I Streptavidin 

  • Catalog # : U0291
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  • Specification
  • Product Description:
  • Fluorescence Dye 594-I with Streptavidin conjugate. Biomolecules conjugated to Fluorescence Dye 594-I exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568nm line of Ar-Kr laser and by the 594nm line of orange He-Ne laser.
  • Recommend Usage:
  • Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Water
  • Storage Instruction:
  • Store the product in desiccated environment at -20°C and avoid from light.
    Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
  • Note:
  • Maximum Excitation/Emission wavelength: 592/614 nm

  • Spectral Equivalent:
  • Alexa Fluor 594, DyLight 594, Texas Red
  • Molar Extinction Coefficient:
  • ≥80,000M-1cm-1
  • Excitation (Max):
  • 592nm
  • Emission (Max):
  • 614nm
  • Solubility:
  • Water
  • Regulation Status:
  • For research use only (RUO)
  • Publication Reference
  • 1.
  • Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.
    Takafumi Kato, Giorgia Radicioni, Micah J Papanikolas, Georgi V Stoychev, Matthew R Markovetz, Kazuhiro Aoki, Melody Porterfield, Kenichi Okuda, Selene M Barbosa Cardenas, Rodney C Gilmore, Cameron B Morrison, Camille Ehre, Kimberlie A Burns, Kristen K White, Tara A Brennan, Henry P Goodell, Holly Thacker, Henry T Loznev, Lawrence J Forsberg, Takahide Nagase, Michael Rubinstein, Scott H Randell, Michael Tiemeyer, David B Hill, Mehmet Kesimer, Wanda K O'Neal, Stephen T Ballard, Ronit Freeman, BriSci Adv. 2022 Apr;8(13):eabm9718. doi: 10.1126/sciadv.abm9718. Epub 2022 Apr 1.
  • Applications
  • Conjugation
  • Application Image
  • Conjugation
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