Fluorescent Dye 594-I Streptavidin
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Maximum Excitation/Emission wavelength: 592/614 nm
Maximum Excitation/Emission wavelength: 592/614 nm
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Specification
Product Description
Fluorescence Dye 594-I with Streptavidin conjugate. Biomolecules conjugated to Fluorescence Dye 594-I exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568nm line of Ar-Kr laser and by the 594nm line of orange He-Ne laser.
Spectral Equivalent
Alexa Fluor 594, DyLight 594, Texas Red
Molar Extinction Coefficient
≥80,000M-1cm-1
Excitation (Max)
592nm
Emission (Max)
614nm
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 592/614 nm
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Applications
Conjugation
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Publication Reference
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Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.
Takafumi Kato, Giorgia Radicioni, Micah J Papanikolas, Georgi V Stoychev, Matthew R Markovetz, Kazuhiro Aoki, Melody Porterfield, Kenichi Okuda, Selene M Barbosa Cardenas, Rodney C Gilmore, Cameron B Morrison, Camille Ehre, Kimberlie A Burns, Kristen K White, Tara A Brennan, Henry P Goodell, Holly Thacker, Henry T Loznev, Lawrence J Forsberg, Takahide Nagase, Michael Rubinstein, Scott H Randell, Michael Tiemeyer, David B Hill, Mehmet Kesimer, Wanda K O'Neal, Stephen T Ballard, Ronit Freeman, Bri
Science Advances 2022 Apr; 8(13):eabm9718.
Application：IF, Biotynlated antibody.
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Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.
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