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Last updated: 2016/12/4

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Fluorescent Dye 540-M Streptavidin

  • Catalog # : U0287
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  • Specification
  • Product Description:
  • Fluorescence Dye 540-M with Streptavidin conjugate. Fluorescent Dye 540-M may have less spillover effect than Pacific Orange due to its slightly sharper emission. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
  • Recommend Usage:
  • Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 g/mL is sufficient.
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Water
  • Storage Instruction:
  • Store the product in desiccated environment at -20°C and avoid from light.
    Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
  • Note:
  • Maximum Excitation/Emission wavelength: 405/537 nm

  • Spectral Equivalent:
  • Krome Orange, Pacific Orange
  • Molar Extinction Coefficient:
  • ≥18,000M-1cm-1
  • Excitation (Max):
  • 405nm
  • Emission (Max):
  • 537nm
  • Recommended Excitation:
  • Violet (405nm)
  • Recommended Filter Sets:
  • Pacific Orange Filter
  • Solubility:
  • Water
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Conjugation
  • Flow Cytometry
  • Application Image
  • Conjugation
  • Flow Cytometry
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