Fluorescence Dye 540-M with Streptavidin conjugate. Fluorescent Dye 540-M may have less spillover effect than Pacific Orange due to its slightly sharper emission. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 g/mL is sufficient. The optimal working dilution should be determined by the end user.
Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
Maximum Excitation/Emission wavelength: 405/537 nm