Fluorescent Dye 532-I Streptavidin
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Specification
Product Description
Fluorescence Dye 532-I with Streptavidin conjugate.
Spectral Equivalent
Alexa Fluor 532
Molar Extinction Coefficient
≥80,000M-1cm-1
Excitation (Max)
542nm
Emission (Max)
558nm
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 542/558 nm
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Applications
Conjugation
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Publication Reference
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Micro and nanotechnology for early diagnosis and detection of rheumatic diseases-molecular markers.
Marulli E, Aloisi A, Giuseppe PD, Rinaldi R.
BioChip Journal 2016 Oct; 10:189.
Application:IF, Human, purified TNF-α.
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Micro and nanotechnology for early diagnosis and detection of rheumatic diseases-molecular markers.
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