Fluorescent Dye 510-M Streptavidin
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Maximum Excitation/Emission wavelength: 414/508 nm
Maximum Excitation/Emission wavelength: 414/508 nm
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Specification
Product Description
Fluorescence Dye 510-M with Streptavidin conjugate. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
Spectral Equivalent
AmCyan
Molar Extinction Coefficient
≥20,000M-1cm-1
Excitation (Max)
414nm
Emission (Max)
508nm
Recommended Excitation
Violet (405nm)
Recommended Filter Sets
FITC Filter, 525/20 nm Filer
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 414/508 nm
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Applications
Conjugation
Flow Cytometry
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