Fluorescence Dye 488-I with Streptavidin conjugate. Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable. Additionally, the fluorescence of Fluorescence Dye 488-I is not affected by pH between 4-10. Compared to Alexa Fluor 488, "Fluorescence Dye 488-I of single isomer has much hihger purity, bringing better performance consistancy from batch to batch.
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient. The optimal working dilution should be determined by the end user.
Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
Maximum Excitation/Emission wavelength: 491/514 nm