Fluorescent Dye 405-I Streptavidin
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Maximum Excitation/Emission wavelength: 401/420 nm
Maximum Excitation/Emission wavelength: 401/420 nm
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Specification
Product Description
Fluorescence Dye 405-I with Streptavidin conjugate. Protein conjugates prepared with Fluorescence Dye 405-I are bright with fluorescence unaffected by pH in the physiological range (pH 4-10). Fluorescence Dye 405-I labeled conjugates are excellent violet laser reagents for flow cytometry research.
Spectral Equivalent
Alexa Fluor 405, DyLight 405
Molar Extinction Coefficient
≥20,000M-1cm-1
Excitation (Max)
401nm
Emission (Max)
420nm
Solubility
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1-20 ug/mL is sufficient.
The optimal working dilution should be determined by the end user.Storage Buffer
Water
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.Note
Maximum Excitation/Emission wavelength: 401/420 nm
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Applications
Conjugation
Flow Cytometry
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