Fluorescent Dye 700-I Hydrazide
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Specification
Product Description
The Fluorescent Dye 700-I have fluorescence excitation and emission maxima of ~690 nm and ~710 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor 700 labeling dyes. Fluorescent Dye 700-I Hydrazide is reasonably stable and shows good reactivity and selectivity with carbonyl group (such as aldehyde). The Fluorescent Dye 700-I Hydrazide can be used for labeling cells. In addition, they are widely used to modify water-soluble biopolymers (such as proteins) through the formation of Schiff Base. These dyes are used for modifications of carbohydrates, glycoproteins and nucleic acids that are first periodate-oxidized to introduce aldehydes or ketones into the biopolymers for subsequent amination. The combination of periodate oxidation with amination provides an effective way for site-selective modifications of biopolymers.
Spectral Equivalent
Alexa Fluor 700
Molar Extinction Coefficient
220000 cm-1M-1
Excitation (Max)
690 nm
Emission (Max)
713 nm
Quantum Yield
0.23
Molecular Weight
1020.97 g/mol
Solvent
Water
Regulation Status
For research use only (RUO)
Recommend Usage
Labeling of biological molecules, e.g. antibody, protein, for fluorescence imaging and other fluorescence-based biochemical analysis.
Storage Instruction
Store the product in desiccated environment at -20°C and avoid from light.
Note
Maximum Excitation/Emission wavelength
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Applications
Conjugation
Flow Cytometry
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