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Fluorescent Dye 550-Z PDA

  • Catalog # : U0123
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  • Specification
  • Product Description:
  • Fluorescent dye derived from trimethine cyanine with an intrinsic neutral charge. Forms a reversible disulfide bond with sulfhydryl groups at pH up to 6.5.
  • Storage Instruction:
  • Store in the dark at -20°C and keep under argon.
  • Note:
  • Maximum Excitation/Emission wavelength: 550/566 nm (in CH3OH)

  • Features:
  • Due to its extreme selectivity, the amine-cross labeling is avoided. The pyridyl disulfide bond can be cleavaged by TCEP (tris-(2-carboxyethyl)phosphine hydrochloride) and DTT (1,4-dithiothreitol). Suitable for 2D-DIGE (Two-dimensional difference gel electrophoresis) in saturation labeling applications, the intrinsic neutral charge does not alter the pI (isoelectric point) of the biomolecule after labeling on the cysteine groups.
  • Chemical Name:
  • 2-((1E,3E)-3-(3,3-dimethyl-1-(5-(3-(pyridin-2-disulfanyl)propanamido)pentyl)indolin-2-ylidene)prop-1-enyl)-1-ethyl-3,3-dimethyl-3H-indolium-5-sulfonate
  • Chemical Formula:
  • C38H46N4O4S3
  • MW:
  • 718.99 g/mol
  • Molar Extinction Coefficient:
  • 150000 M-1cm-1 (CH3OH)
  • Excitation (Max):
  • 550 nm (CH3OH)
  • Emission (Max):
  • 566 nm (CH3OH)
  • Recommended Excitation:
  • 532 nm Nd:YAG, 532 nm green diode and 543 nm green HeNe.
  • Recommended Filter Sets:
  • TRITC
  • Solubility:
  • Dichloromethane, methanol, DMSO, DMF and acetonitrile.
    Slightly in water.
  • Purity:
  • ≥ 95%
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Conjugation
  • Application Image
  • Conjugation
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