SOD2 recombinant monoclonal antibody, clone 10F6
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and rat SOD2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human SOD2.
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: HepG2 whole cell lysate; Lane 4: U87 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: Rat brain tissue; Lane 7: Brain tissue; Lane 8: Liver tissue.Immunohistochemistry
Immunohistochemistry image of SOD2 recombinant monoclonal antibody, clone 10F6 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SOD2
Entrez GeneID
6648Protein Accession#
P04179Gene Name
SOD2
Gene Alias
IPO-B, MNSOD, Mn-SOD
Gene Description
superoxide dismutase 2, mitochondrial
Omim ID
147460Gene Ontology
HyperlinkGene Summary
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
Mn superoxide dismutase|OTTHUMP00000017530|OTTHUMP00000017531|indophenoloxidase B|manganese superoxide dismutase|manganese-containing superoxide dismutase|mangano-superoxide dismutase
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Interactome
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Disease
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