Cd276 recombinant monoclonal antibody, clone MJ18
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Cd276 recombinant monoclonal antibody, clone MJ18 (Cat # RAB03066).
Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of RAB03066 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Cd276 recombinant monoclonal antibody, clone MJ18 (Cat# RAB03066).
Binding curve of RAB03067 to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using 24-552. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd276.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against Cd276-Ig, consisting of the extracellular domain (aa1–242 of mouse Cd276) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco).
Reactivity
Mouse
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG kappa
Recommend Usage
Blocking
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Cd276 recombinant monoclonal antibody, clone MJ18 (Cat # RAB03066).
Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of RAB03066 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Cd276 recombinant monoclonal antibody, clone MJ18 (Cat# RAB03066).
Binding curve of RAB03067 to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using 24-552. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used.Blocking
Flow Cytometry
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Gene Info — Cd276
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