Lgals9 recombinant monoclonal antibody, clone RG9-35
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Lgals9 recombinant monoclonal antibody, clone RG9-35 (Cat # RAB03480).
Immunofluorescence analysis of paraformaldehyde RAW264.7 cells fixed on Shi-fix™ coverslips stained with the chimeric r version of RAB03480 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03480
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of NIH3T3 cells with Lgals9 recombinant monoclonal antibody, clone RG9-35 (Cat # RAB03480).
The NIH3T3 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control-black line) or the r1 version of RAB03480 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Lgals9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to mouse Lgals9.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Blocking
Flow Cytometry
Functional Study
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Lgals9 recombinant monoclonal antibody, clone RG9-35 (Cat # RAB03480).
Immunofluorescence analysis of paraformaldehyde RAW264.7 cells fixed on Shi-fix™ coverslips stained with the chimeric r version of RAB03480 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03480
(B) DAPI
(C) Merged channels
(D) Isotype controlFunctional Study
Blocking
Flow Cytometry
Flow cytometric analysis of NIH3T3 cells with Lgals9 recombinant monoclonal antibody, clone RG9-35 (Cat # RAB03480).
The NIH3T3 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control-black line) or the r1 version of RAB03480 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Lgals9
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