Flavivirus group antigen recombinant monoclonal antibody, clone D1-4G2-4-15 (4G2)
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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Denge Virus-Like Particles with Flavivirus group antigen recombinant monoclonal antibody, clone D1-4G2-4-15 (4G2) (Cat # RAB03450).
Dengue Virus Serotype 2 VLPs were coated onto a plate at 5 µg/mL. RAB03450 added to plate in a 3-fold serial dilution starting at 1000 ng/mL. Detection performed using HRP labelled goat anti-mouse IgG (AbD Serotec, 0300-0108P).
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against Flavivirus group antigen.
Antibody Species
Mouse
Immunogen
Original antibody is raised against Dengue Virus type 2 antigens.
Reactivity
Viruses
Form
Liquid
Isotype
IgG2a kappa
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Neutralization
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Denge Virus-Like Particles with Flavivirus group antigen recombinant monoclonal antibody, clone D1-4G2-4-15 (4G2) (Cat # RAB03450).
Dengue Virus Serotype 2 VLPs were coated onto a plate at 5 µg/mL. RAB03450 added to plate in a 3-fold serial dilution starting at 1000 ng/mL. Detection performed using HRP labelled goat anti-mouse IgG (AbD Serotec, 0300-0108P).Flow Cytometry
Neutralization
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