Cd8b1 recombinant monoclonal antibody, clone YTS 156.7.7
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More Files
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Flow Cytometry
Flow cytometric analysis of Mouse thymocytes with Cd8b1 recombinant monoclonal antibody, clone YTS 156.7.7 (Cat # RAB03356).
Mouse thymocytes were stained with unimmunized r antibody (isotype control-black line) or the r version of RAB03356 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer.
Flow Cytometry
Flow cytometric analysis of mouse lymphocytes with Cd8b1 recombinant monoclonal antibody, clone YTS 156.7.7 (Cat # RAB03356).
Mouse lymphocytes were stained with an anti-CD44 control (isotype control-panel A) or the r version of RAB03356 (panel B) at a concentration of 1 ug/mL for 30 mins at RT. After washing the bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd8b1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse Cd8b1 (lyt-3).
Reactivity
Mouse
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG, kappa
Recommend Usage
Flow Cytometry
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Flow Cytometry
Flow cytometric analysis of Mouse thymocytes with Cd8b1 recombinant monoclonal antibody, clone YTS 156.7.7 (Cat # RAB03356).
Mouse thymocytes were stained with unimmunized r antibody (isotype control-black line) or the r version of RAB03356 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer.Flow Cytometry
Flow cytometric analysis of mouse lymphocytes with Cd8b1 recombinant monoclonal antibody, clone YTS 156.7.7 (Cat # RAB03356).
Mouse lymphocytes were stained with an anti-CD44 control (isotype control-panel A) or the r version of RAB03356 (panel B) at a concentration of 1 ug/mL for 30 mins at RT. After washing the bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Cd8b1
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