Pvr recombinant monoclonal antibody, clone 3F1
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More Files
- More Functions
Western Blot
Western blot analysis of mouse thymus lysate with Pvr recombinant monoclonal antibody, clone 3F1 (Cat # RAB03166).
Immunofluorescence
Immunofluorescent staining of RAW 264.7 cells with Pvr recombinant monoclonal antibody, clone 3F1 (Cat # RAB03166).
Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric r version of RAB03166 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03166- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Pvr recombinant monoclonal antibody, clone 3F1 (Cat # RAB03166).
Binding curve of RAB03166 to recombinant mouse CD155 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse CD155 Fc-Fusion Protein (Pr00231-1.9; Antibody) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 ng/ml was performed using RAB03166. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Pvr.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of mouse thymus lysate with Pvr recombinant monoclonal antibody, clone 3F1 (Cat # RAB03166).Immunofluorescence
Immunofluorescent staining of RAW 264.7 cells with Pvr recombinant monoclonal antibody, clone 3F1 (Cat # RAB03166).
Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric r version of RAB03166 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03166- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Pvr recombinant monoclonal antibody, clone 3F1 (Cat # RAB03166).
Binding curve of RAB03166 to recombinant mouse CD155 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse CD155 Fc-Fusion Protein (Pr00231-1.9; Antibody) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 ng/ml was performed using RAB03166. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.Flow Cytometry
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Gene Info — Pvr
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