FUBP1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of mouse spleen (A), K562 (B), rat brain (C), Hela (D) whole cell lysates with FUBP1 recombinant monoclonal antibody (Cat # RAB02501).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using FUBP1 recombinant monoclonal antibody (Cat # RAB02501). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.57). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescence
Immunofluorescent analysis of HEK293 cells with FUBP1 recombinant monoclonal antibody (Cat # RAB02501). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FUBP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human FUBP1.
Theoretical MW (kDa)
70
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of FUBP1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Supplied Product
Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of mouse spleen (A), K562 (B), rat brain (C), Hela (D) whole cell lysates with FUBP1 recombinant monoclonal antibody (Cat # RAB02501).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using FUBP1 recombinant monoclonal antibody (Cat # RAB02501). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.57). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HEK293 cells with FUBP1 recombinant monoclonal antibody (Cat # RAB02501). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — FUBP1
Entrez GeneID
8880Protein Accession#
Q96AE4Gene Name
FUBP1
Gene Alias
FBP, FUBP
Gene Description
far upstream element (FUSE) binding protein 1
Omim ID
603444Gene Ontology
HyperlinkGene Summary
This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq
Other Designations
DNA helicase V|FUSE-binding protein|OTTHUMP00000038483|far upstream element binding protein|far upstream element-binding protein
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