GCLC recombinant monoclonal antibody, clone R08-2F5
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GCLC.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human GCLC.
Theoretical MW (kDa)
Calculated MW: 73 kD
Reactivity
Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: rat brain, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with GCLC recombinant monoclonal antibody, clone R08-2F5 (Cat # RAB02262).Immunoprecipitation
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Gene Info — GCLC
Entrez GeneID
2729Protein Accession#
P48506Gene Name
GCLC
Gene Alias
GCS, GLCL, GLCLC
Gene Description
glutamate-cysteine ligase, catalytic subunit
Gene Ontology
HyperlinkGene Summary
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. [provided by RefSeq
Other Designations
gamma-glutamylcysteine synthetase
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Interactome
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Pathway
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Disease
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