GPD2 recombinant monoclonal antibody, clone R02-5B9
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: 3T3 and Lane 2: Hela lysates with GPD2 recombinant monoclonal antibody, clone R02-5B9 (Cat # RAB02240).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GPD2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human GPD2.
Theoretical MW (kDa)
Calculated MW: 81 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: 3T3 and Lane 2: Hela lysates with GPD2 recombinant monoclonal antibody, clone R02-5B9 (Cat # RAB02240).Immunoprecipitation
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Gene Info — GPD2
Entrez GeneID
2820Protein Accession#
P43304Gene Name
GPD2
Gene Alias
GDH2, GPDM, mGPDH
Gene Description
glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
Gene Ontology
HyperlinkGene Summary
Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM
Other Designations
mitochondrial glycerophosphate dehydrogenase
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Interactome
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Pathway
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