H2BC12 recombinant monoclonal antibody, clone R07-8K4
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with H2BC12 recombinant monoclonal antibody, clone R07-8K4 (Cat # RAB02223).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with H2BC12 recombinant monoclonal antibody, clone R07-8K4 (Cat # RAB02223). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human H2BC12.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human H2BC12.
Theoretical MW (kDa)
Calculated MW: 14 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with H2BC12 recombinant monoclonal antibody, clone R07-8K4 (Cat # RAB02223).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with H2BC12 recombinant monoclonal antibody, clone R07-8K4 (Cat # RAB02223). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — HIST1H2BK
Entrez GeneID
85236Protein Accession#
O60814Gene Name
HIST1H2BK
Gene Alias
H2B/S, H2BFAiii, H2BFT, MGC131989
Gene Description
histone cluster 1, H2bk
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq
Other Designations
H2B histone family, member T|OTTHUMP00000016177|histone 1, H2bk|histone family member
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Interactome
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Pathway
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