NCK2 recombinant monoclonal antibody, clone R01-3C6
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NCK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NCK2.
Theoretical MW (kDa)
Calculated MW: 43 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of Hela with NCK2 recombinant monoclonal antibody, clone R01-3C6 (Cat # RAB02077). (A) NCK2 (green) and (B) DAPI (blue).Immunofluorescence
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Gene Info — NCK2
Entrez GeneID
8440Protein Accession#
O43639Gene Name
NCK2
Gene Alias
GRB4, NCKbeta
Gene Description
NCK adaptor protein 2
Omim ID
604930Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
SH2/SH3 adaptor protein NCK-beta|noncatalytic region of tyrosine kinase, beta
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Interactome
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