VAMP2 recombinant monoclonal antibody, clone R04-7A5
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human VAMP2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human VAMP2.
Theoretical MW (kDa)
Calculated MW: 13 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of rat brain lysates with VAMP2 recombinant monoclonal antibody, clone R04-7A5 (Cat # RAB01838).Immunofluorescence
Immunoprecipitation
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Gene Info — VAMP2
Entrez GeneID
6844Protein Accession#
P63027Gene Name
VAMP2
Gene Alias
FLJ11460, SYB2, VAMP-2
Gene Description
vesicle-associated membrane protein 2 (synaptobrevin 2)
Omim ID
185881Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq
Other Designations
synaptobrevin 2|vesicle associated membrane protein 2
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