POLD1 recombinant monoclonal antibody, clone R05-3H1
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More Files
- More Functions
Western Blot
Western blot analysis of POLD1 in K562, C6, 3T3, Hela lysates using human POLD1 recombinant monoclonal antibody, clone R05-3H1 (Cat # RAB01717).
Immunocytochemistry
Immunocytochemistry analysis of POLD1 (green) in K562 using human POLD1 recombinant monoclonal antibody, clone R05-3H1 (Cat # RAB01717), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human DNA Polymerase delta, catalytic subunit.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human DNA Polymerase delta, catalytic subunit
Theoretical MW (kDa)
Calculated MW: 124 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of POLD1 in K562, C6, 3T3, Hela lysates using human POLD1 recombinant monoclonal antibody, clone R05-3H1 (Cat # RAB01717).Immunocytochemistry
Immunocytochemistry analysis of POLD1 (green) in K562 using human POLD1 recombinant monoclonal antibody, clone R05-3H1 (Cat # RAB01717), and DAPI(blue). -
Gene Info — POLD1
Entrez GeneID
5424Protein Accession#
P28340Gene Name
POLD1
Gene Alias
CDC2, POLD
Gene Description
polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Omim ID
174761Gene Ontology
HyperlinkGene Summary
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM
Other Designations
CDC2 homolog|DNA-directed polymerase delta 1|polymerase (DNA directed), delta 1, catalytic subunit (125kD)
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Interactome
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Pathway
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Disease
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