ALDOA recombinant monoclonal antibody, clone R06-6A3
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More Files
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Western Blot
Western blot analysis of Aldolase in K562, rat Brain, C6, 3T3, Hela lysates using human Aldolase recombinant monoclonal antibody, clone R06-6A3 (Cat # RAB01620).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Aldolase.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Aldolase
Theoretical MW (kDa)
Calculated MW: 39 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Aldolase in K562, rat Brain, C6, 3T3, Hela lysates using human Aldolase recombinant monoclonal antibody, clone R06-6A3 (Cat # RAB01620).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
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Gene Info — ALDOA
Entrez GeneID
226Protein Accession#
P04075Gene Name
ALDOA
Gene Alias
ALDA, MGC10942, MGC17716, MGC17767
Gene Description
aldolase A, fructose-bisphosphate
Omim ID
103850Gene Ontology
HyperlinkGene Summary
This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein. [provided by RefSeq
Other Designations
aldolase A|fructose-1,6-bisphosphate triosephosphate-lyase|fructose-bisphosphate aldolase A
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Fructose and mannose metabolism
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