EEF1A1 recombinant monoclonal antibody, clone R04-3H4
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More Files
- More Functions
Western Blot
Western blot analysis of eEF1A1/EFTu in U2OS, C6 lysates using eEF1A1/EFTu antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using eEF1A1/EFTu antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EEF1A1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human EEF1A1.
Theoretical MW (kDa)
Calculated MW: 50 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of eEF1A1/EFTu in U2OS, C6 lysates using eEF1A1/EFTu antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using eEF1A1/EFTu antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
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Gene Info — EEF1A1
Entrez GeneID
1915Protein Accession#
P68104Gene Name
EEF1A1
Gene Alias
CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1
Gene Description
eukaryotic translation elongation factor 1 alpha 1
Omim ID
130590Gene Ontology
HyperlinkGene Summary
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq
Other Designations
CTCL tumor antigen|EF1a-like protein|OTTHUMP00000016736|OTTHUMP00000063804|OTTHUMP00000063805|cervical cancer suppressor 3|elongation factor 1 alpha subunit|elongation factor 1-alpha|elongation factor Tu|eukaryotic translation elongation factor 1 alpha 1-
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